DEVELOPMENT OF AN AUTOMATED IMMUNOTURBIDIMETRIC FERRITIN ASSAY PDF

Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.

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Ferritin is protein that has a central role in the storage of iron. The immunopurified antibody showed a similar trend, having a covalently coupled adsorption isotherm according to that of the nonimmunopurified antibody in the range studied 0.

Principle of Test Method: In addition, we present a new ferriitin of sequential covalent coupling of IgG and bovine serum albumin BSA that improves the reagent colloidal stability and could eliminate most of the drawbacks of the light-scattering immunoassays, such as nonspecific agglutination. Development of an automated immunoturbidimetric ferritin assay. Biomarker Menu Search Search by Keyword: Posttreatment with additives BSA, surfactants has been proposed 13 14although it reduces IgG charge and reactivity.

Increased serum C-reactive protein levels by immunonephelometry in patients with rapidly destructive hip osteoarthritis. Turbid and lipemic samples develipment an important interference which could be avoided by pretreating those samples prior to measurement. Download figure Open in new tab. Eur J Pediatr ; Clin Chem ; 42 Suppl 6: CRP measurements have been used for many years in the management of a variety of clinical situations, such as bacterial infections, ischemic necrosis of tissue, and active inflammatory conditions 1.

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A new method of measuring C-reactive protein, with a low limit of detection, suitable for risk assessment of coronary heart disease.

The turbidimetric method affords a measuring range of 0. Clin Chem ; 45 Suppl 6: Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency.

We have developed a new procedure for turbidimetric measurement of ferritin concentration in human serum, based on latex microparticle agglutination technology. The maximum adsorption plateau is achieved at a polyclonal antibody concentrations of 3. develoopment

Development of an automated immunoturbidimetric ferritin assay.

View inline View popup. We describe a microparticle reagent that we use in an optimized turbidimetric procedure with immunopurified polyclonal antibodies against CRP. Clinical Chemistry Nov46 11. A microparticle immunothrbidimetric of nm and a wavelength of nm were selected as the best choice because of the high slope and wide dynamic range showed. Download this assay page in pdf format. After elution, the released protein was measured using the BCA procedure.

Evaluation of four automated high-sensitivity C-reactive protein methods: Biological or Clinical Significance: Coated microparticles were automatically mixed with undiluted sample and the resulting absorbance due to agglutination was measured at nm.

Development of an automated immunoturbidimetric ferritin assay.

We chose this microparticle reagent, with a coverage of 0. Ferritin determinations are widely used for monitoring renal anemia when iron utilization and distribution disorders are present during therapy with erythropoietin. For the immunopurified anti-CRP antibodies, we used a modified procedure consisting of two sequential covalent coupling steps.

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Jump to section Article Acknowledgments References. The determination of ferritin is useful in diagnosis of immunoturbidlmetric in iron metabolism, monitoring iron therapy, ascertaining the iron reserves in groups at risk and in the differential diagnosis of anemias. Thus, a compromise between sensitivity and measurement range is demanded.

The most striking feature in Fig.

The microparticle reagent response of the different antibodies to increasing CRP concentrations can be seen in Fig. Rev Rheum Engl Ed ; Variability in the measurement of C-reactive protein in healthy subjects: Test Directory Entire Site. It is also used to distinguish between hypoferric anemia and hypochromic anemia chronic infection and tumor developmentt, sideroblastic anemia or thalassemia.

Sn use of serum ferritin encompasses prelatent and latent iron deficiency as well as iron overloading. Ann Clin Biochem ; Clin Chem ; A rapid and sensitive automated light scattering immunoassay for serum C-reactive protein and the definition of a reference range in healthy blood donors.

Recent reports show median values in healthy controls of 0.

We monitored the reaction kinetics at pH values of 6. The value of acute phase protein measurements in clinical practice. Carboxilated latex particles nm in diameter were covalently coupled with immunopurified F ab’ 2 fragments of anti-ferritin IgG antibodies. Colloidal stability and immunoreactivity. The procedure has been automated using the Falcor analyzer.